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  3. A300-788A
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Rabbit anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified

Catalogue number:
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A300-788A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (200 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£464.00
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Estimated delivery Fri 3 May
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Applications:

WB, IHC, Flow Cyt

Antibody Host:

Rabbit

Target:

MCM2, Phospho (S40/S41)

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

surrounding serine 40/41

Alternative Names:

D3S3194; MITOTIN; minichromosome maintenance protein 2 homolog; minichromosome maintenance deficient 2 (mitotin); nuclear protein BM28; DFNA70; cell devision cycle-like 1; CDCL1; cdc19; CCNL1; BM28; DNA replication licensing factor MCM2; cyclin-like 1

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Product Description:

Rabbit anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified - 100 µl (200 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human Phospho MCM2 (S40/S41
Detection of human Phospho MCM2 (S40/S41) by western blot. Samples: Whole cell lysate (50 µg) from asynchronous HEK293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S40/S41) antibody (Cat. No. A300-788A) used at 0.1 µg/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody (Cat. No. A300-191A) at 0.1 µg/ml. Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).
Detection of human Phospho MCM2 (S40/S41
Detection of human Phospho MCM2 (S40/S41) by immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) (Cat. No. A300-788A Lot 2) used at a dilution of 1:200 (1 µg/ml). Detection: DAB
Flow cytometric analysis of phospho-MCM2
Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.1 µg/ml anti-phospho-MCM2 (pS40/41) [A300-788A]in a 150 µl volume. DNA content was simultaneously analyzed via PI stain.
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