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Rabbit anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified - A300-788A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified
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Size: 20 µg
£322.00
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Catalogue number:
A300-788A
Name:
Rabbit anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Phospho MCM2 (S40/S41) immobilized on solid support.Immunogen was a synthetic di-phosphorylated peptide, which represented a portion of human minichromosomal maintenance deficient 2 surrounding Serines 40 and 41 according to the numbering given in entry NP_004517.2 (GeneID 4171).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IHC, F

Product Type:
Antibodies
Alternative Names:
BM28, CCNL1, cdc19, CDCL1, cell devision cycle-like 1, cyclin-like 1, D3S3194, DFNA70, DNA replication licensing factor MCM2, minichromosome maintenance deficient 2 (mitotin), minichromosome maintenance protein 2 homolog, MITOTIN, nuclear protein BM28
Species Reactivity:
Human
Immunogen:
Surrounding serine 40/41
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Phospho MCM2 (S40/S41) by western blot. Samples: Whole cell lysate (50 µg) from asynchronous HEK293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S40/S41) antibody (Cat. No. A300-788A) used at 0.1 µg/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody (Cat. No. A300-191A) at 0.1 µg/ml. Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).

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Detection of human Phospho MCM2 (S40/S41) by immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) (Cat. No. A300-788A Lot 2) used at a dilution of 1:200 (1 µg/ml). Detection: DAB

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Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.1 µg/ml anti-phospho-MCM2 (pS40/41) [A300-788A]in a 150 µl volume. DNA content was simultaneously analyzed via PI stain.

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