Antibody was affinity purified using an epitope specific to Phospho MCM2 (S40/S41) immobilized on solid support. Immunogen was a synthetic di-phosphorylated peptide, which represented a portion of human minichromosomal maintenance deficient 2 surrounding Serines 40 and 41 according to the numbering given in entry NP_004517.2 (GeneID 4171). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BM28, CCNL1, cdc19, CDCL1, cell devision cycle-like 1, cyclin-like 1, D3S3194, DFNA70, DNA replication licensing factor MCM2, minichromosome maintenance deficient 2 (mitotin), minichromosome maintenance protein 2 homolog, MITOTIN, nuclear protein BM28
surrounding serine 40/41
2 - 8°C
Detection of human Phospho MCM2 (S40/S41) by western blot. Samples: Whole cell lysate (50 µg) from asynchronous HEK293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S40/S41) antibody (Cat. No. A300-788A) used at 0.1 µg/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody (Cat. No. A300-191A) at 0.1 µg/ml. Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).
Detection of human Phospho MCM2 (S40/S41) by immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho MCM2 (S40/S41) (Cat. No. A300-788A Lot 2) used at a dilution of 1:200 (1 µg/ml). Detection: DAB