Antibody was affinity purified using an epitope specific to Visfatin immobilized on solid support. The epitope recognized by A300-778A maps to a region between residue 400 and 450 of human Visfatin (PBEF/NAMPT) using the numbering given in entry NP_005737.1 (GeneID 10135). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
1110035O14Rik, NAmPRTase, Nampt, nicotinamide phosphoribosyltransferase, PBEF, PBEF1, pre-B cell-enhancing factor, pre-B-cell colony-enhancing factor 1, VF, VISFATIN
between 400 and 450
2 - 8°C
Detection of human Visfatin by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Visfatin antibody BL2866 (Cat. No. A300-778A) used for IP at 3 µg/mg lysate. Visfatin was also immunoprecipitated by rabbit anti-Visfatin antibody BL2867 (Cat. No. A300-779A). For blotting immunoprecipitated Visfatin, BL2867 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.