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Rabbit anti-Visfatin Antibody, Affinity Purified

Catalogue number:
A300-778A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to Visfatin immobilized on solid support. The epitope recognized by A300-778A maps to a region between residue 400 and 450 of human Visfatin (PBEF/NAMPT) using the numbering given in entry NP_005737.1 (GeneID 10135). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP
Product Type:

Antibodies

Alternative Names:

1110035O14Rik, NAmPRTase, Nampt, nicotinamide phosphoribosyltransferase, PBEF, PBEF1, pre-B cell-enhancing factor, pre-B-cell colony-enhancing factor 1, VF, VISFATIN

Species Reactivity:

Human

Immunogen:

between 400 and 450

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Visfatin by western b

Detection of human Visfatin by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Visfatin antibody BL2866 (Cat. No. A300-778A) used for IP at 3 µg/mg lysate. Visfatin was also immunoprecipitated by rabbit anti-Visfatin antibody BL2867 (Cat. No. A300-779A). For blotting immunoprecipitated Visfatin, BL2867 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.