Rabbit anti-MIS12 Antibody, Affinity Purified - A300-776A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to MIS12 immobilized on solid support.The epitope recognized by A300-776A maps to a region between residue 150 and the C-terminus (residue 205) of human MIS12 homolog using the numbering given in entry NP_076944.1 (GeneID 79003).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC
Detection of human MIS12 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIS12 antibody A300-776A (lot A300-776A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human MIS12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIS12 antibody A300-776A (lot A300-776A-2) used for IP at 3 µg per reaction. MIS12 was also immunoprecipitated by rabbit anti-MIS12 antibody A300-775A. For blotting immunoprecipitated MIS12, A300-776A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human MIS12 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MIS12 (Cat. No. A300-776A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
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