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Rabbit anti-HEC1 Antibody, Affinity Purified

Catalogue number:
A300-770A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to HEC1 immobilized on solid support. The epitope recognized by A300-770A maps to a region between residue 1 and 50 of human Highly Expressed in Cancer 1 (Kinetochore Associated 2) using the numbering given in entry NP_006092.1 (GeneID 10403). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP
Detection of human HEC1 by western blot
Product Type:

Antibodies

Alternative Names:

HEC, HEC1, highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, HsHec1, hsNDC80, kinetochore associated 2, kinetochore protein Hec1, kinetochore protein NDC80 homolog, kinetochore-associated protein 2, KNTC2, NDC

Species Reactivity:

Human

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human HEC1 by western blot

Detection of human HEC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HEC1 antibody BL3440 (Cat. No. A300-770A) used for IP at 3 µg/mg lysate. HEC1 was also immunoprecipitated by rabbit anti-HEC1 antibodies BL3441 and BL3443 (Cat. No. A300-771A). For blotting immunoprecipitated HEC1, BL3443 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.