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Rabbit anti-HEC1 Antibody, Affinity Purified - A300-770A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-HEC1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-770A
Name:
Rabbit anti-HEC1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to HEC1 immobilized on solid support.The epitope recognized by A300-770A maps to a region between residue 1 and 50 of human Highly Expressed in Cancer 1 (Kinetochore Associated 2) using the numbering given in entry NP_006092.1 (GeneID 10403).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

IP

Product Type:
Antibodies
Alternative Names:
HEC, HEC1, highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, HsHec1, hsNDC80, kinetochore associated 2, kinetochore protein Hec1, kinetochore protein NDC80 homolog, kinetochore-associated protein 2, KNTC2, NDC
Species Reactivity:
Human
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human HEC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HEC1 antibody BL3440 (Cat. No. A300-770A) used for IP at 3 µg/mg lysate. HEC1 was also immunoprecipitated by rabbit anti-HEC1 antibodies BL3441 and BL3443 (Cat. No. A300-771A). For blotting immunoprecipitated HEC1, BL3443 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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