*

Rabbit anti-TORC1 Antibody, Affinity Purified

Catalogue number:
A300-769A
Size:
20 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to TORC1 immobilized on solid support. The epitope recognized by A300-769A maps to a region between residue 600 and the C-terminus (residue 634) of human Transducer of Regulated cAMP Response element-binding protein 1 (CREB Regulated Transcription Coactivator 1) using the numbering given in entry NP_056136.1 (GeneID 23373). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

CREB-regulated transcription coactivator 1, Mam-2, MAML2, Mastermind-like protein 2, MECT1, mucoepidermoid carcinoma translocated protein 1, TORC1, TORC-1, Transducer of CREB protein 1, transducer of regulated cAMP response element-binding protein (CREB)

Species Reactivity:

Human, Mouse

Immunogen:

between 600 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human TORC1 by western blot

Detection of human TORC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TORC1 antibody A300-769A (lot A300-769A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse TORC1 by western blot

Detection of mouse TORC1 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TORC1 antibody A300-769A (lot A300-769A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human TORC1 by western blot

Detection of human TORC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TORC1 antibody A300-769A (lot A300-769A-2) used for IP at 3 µg per reaction. TORC1 was also immunoprecipitated by rabbit anti-TORC1 antibody A300-768A. For blotting immunoprecipitated TORC1, A300-769A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human TORC1 by immunohistoc

Detection of human TORC1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-TORC1 (Cat. No. A300-769A Lot 2) used at a dilution of 1:200 (1µg/ml). Detection: DAB