Antibody was affinity purified using an epitope specific to RAP80 immobilized on solid support. The epitope recognized by A300-764A maps to a region between residue 650 and the C-terminus (residue 719) of human Receptor Associated Protein 80 using the numbering given in entry NP_057374.3 (GeneID 51720). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BRCA1-A complex subunit RAP80, RAP80, receptor-associated protein 80, retinoid X receptor-interacting protein 110, Ubiquitin interaction motif-containing protein 1, X2HRIP110
between 650 and C-term
2 - 8°C
Detection of human RAP80 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5 and 15 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RAP80 antibody BL2840 (Cat. No. A300-764A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). RAP80 was also immunoprecipitated using rabbit anti-RAP80 antibody BL2839 (Cat. No. A300-763A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).