Antibody was affinity purified using an epitope specific to rpL7a/SURF3 immobilized on solid support. The epitope recognized by A300-749A maps to a region between residue 215 and the C-terminus (residue 266) of human Ribosomal Protein L7a using the numbering given in entry NP_000963.1 (GeneID 6130). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
60S ribosomal protein L7a, L7A, large ribosomal subunit protein eL8, PLA-X polypeptide, SURF3, surfeit 3, surfeit locus protein 3, thyroid hormone receptor uncoupling protein, TRUP
between 215 and C-term
2 - 8°C
Detection of human rpL7a/SURF3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-rpL7a/SURF3 antibody A300-749A (lot A300-749A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human rpL7a (SURF3) by western blot and immunoprecipitation/Mass Spectrometry. Samples: A) Whole cell lysate from HeLa (20 and 4 µg), MCF-7 (7; 20µg) and HEK293T (T; 20µg) cells. B) Cytosolic and nuclear extract (10 mg/IP) from HeLa S3 cells. Antibodies: Affinity purified rabbit anti-rpL7a (SURF3) antibody (Cat. No. A300-749A) used for WB at 0.1 µg/ml (A) and for IP at 0.7 µg/mg lysate (B). Detection: Chemiluminescence with an exposure time of 5 sec (A) and vMALDI-LTQ and LC-MS/MS of Coomassie stained bands (B).