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Rabbit anti-rpL7a/SURF3 Antibody, Affinity Purified - A300-749A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-rpL7a/SURF3 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-749A
Name:
Rabbit anti-rpL7a/SURF3 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to rpL7a/SURF3 immobilized on solid support.The epitope recognized by A300-749A maps to a region between residue 215 and the C-terminus (residue 266) of human Ribosomal Protein L7a using the numbering given in entry NP_000963.1 (GeneID 6130).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
60S ribosomal protein L7a, L7A, large ribosomal subunit protein eL8, PLA-X polypeptide, SURF3, surfeit 3, surfeit locus protein 3, thyroid hormone receptor uncoupling protein, TRUP
Species Reactivity:
Human, Mouse
Immunogen:
Between 215 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human rpL7a/SURF3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-rpL7a/SURF3 antibody A300-749A (lot A300-749A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human and mouse rpL7a (SURF3) by immunohistochemistry. Sample: FFPE sections of human colon carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- rpL7a (SURF3) (Cat. No. A300-749A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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Detection of human rpL7a (SURF3) by western blot and immunoprecipitation/Mass Spectrometry. Samples: A) Whole cell lysate from HeLa (20 and 4 µg), MCF-7 (7; 20µg) and HEK293T (T; 20µg) cells. B) Cytosolic and nuclear extract (10 mg/IP) from HeLa S3 cells. Antibodies: Affinity purified rabbit anti-rpL7a (SURF3) antibody (Cat. No. A300-749A) used for WB at 0.1 µg/ml (A) and for IP at 0.7 µg/mg lysate (B). Detection: Chemiluminescence with an exposure time of 5 sec (A) and vMALDI-LTQ and LC-MS/MS of Coomassie stained bands (B).

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