Antibody was affinity purified using an epitope specific to UBCH7 immobilized on solid support. The epitope recognized by A300-737A maps to a region between residue 100 and the C-terminus (residue 154) of human Ubiquitin-Conjugating Enzyme H7 using the numbering given in entry NP_003338.1 (GeneID 7332). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
E2 ubiquitin-conjugating enzyme L3, E2-F1, L-UBC, UBCH7, UbcM4, ubiquitin carrier protein L3, ubiquitin conjugating enzyme E2L 3, ubiquitin-conjugating enzyme E2 L3, ubiquitin-conjugating enzyme E2-F1, ubiquitin-conjugating enzyme UBCH7, ubiquitin-protein
between 100 and C-term
2 - 8°C
Detection of human and mouse UBCH7 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-UBCH7 antibody A300-737A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A and B).