Antibody was affinity purified using an epitope specific to eIF2alpha/EIF2S1 immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. The epitope recognized by A300-721A maps to a region between residue 275 and the C-terminus (residue 315) of human Eukarytic Translation Initiation Factor 2, subunit 1 alpha, 35kDa using the numbering given in entry NP_004085.1 (GeneID 1965).
EIF2, EIF-2, EIF2A, EIF-2A, EIF-2alpha, eIF-2-alpha, eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
between 275 and C-term
2 - 8°C
Detection of human and mouse eIF2alpha/EIF2S1 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF2alpha/EIF2S1 antibody BL3226 (Cat. No. A300-721A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of human and mouse eIF2alpha/EIF2S1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eIF2alpha/EIF2S1 (Cat. No. A300-721A Lot1) used at a dilution of 1:1,000 (0.2µg/ml) and 1:200 (1µg/ml). Detection: DAB