Antibody was affinity purified using an epitope specific to HDAC1 immobilized on solid support. The epitope recognized by A300-712A maps to the N-terminus of human Histone Deacetylase 1 using the numbering given in entry NP_004955.2 (GeneID 3065). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
GON-10, HD1, histone deacetylase 1, reduced potassium dependency, yeast homolog-like 1, RPD3, RPD3L1
2 - 8°C
Detection of human HDAC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HDAC1 antibody BL2607 (Cat. No. A300-712A) used for IP at 3 µg/mg lysate (B). HDAC1 was also immunoprecipitated using rabbit anti-HDAC1 antibody BL2608 (Cat. No. A300-713A) at 3 µg/mg lysate. For blotting immunoprecipitated HDAC1, BL2608 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.