Antibody was affinity purified using an epitope specific to Nucleolin (NCL) immobilized on solid support. The epitope recognized by A300-711A maps to a region between residue 550 and the C-terminus (residue 710) of human Nucleolin using the numbering given in entry NP_005372.2 (GeneID 4691). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
C23, Nsr1, nucleolin, Protein C23
between 550 and C-term
2 - 8°C
Detection of human and mouse Nucleolin by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Nucleolin antibody A300-711A used for WB at 0.02 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Nucleolin was also immunoprecipitated using rabbit anti-Nucleolin antibodies A300-709A and A300-710A. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
Detection of human and mouse Nucleolin by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Nucleolin (Cat. No. A300-711A Lot1) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB
Detection of mouse Nucleolin by immunohistochemistry. Sample: FFPE section of mouse cerebellum. Antibody: Affinity purified rabbit anti- Nucleolin (Cat. No. A302-711A Lot1) used at a dilution of 1:100 (2µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.