Rabbit anti-Nucleolin (NCL) Antibody, Affinity Purified

Catalogue number:
20 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to Nucleolin (NCL) immobilized on solid support. The epitope recognized by A300-710A maps to a region between residue 525 and 575 of human Nucleolin using the numbering given in entry NP_005372.2 (GeneID 4691). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:


Alternative Names:

C23, Nsr1, nucleolin, Protein C23

Species Reactivity:



between 525 and 575

Storage Temp:

2 - 8°C

Antibody Host:


Antibody Isotype:

Whole IgG

Antibody Type:




Detection of human Nucleolin by western

Detection of human Nucleolin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Nucleolin antibody BL3214 (Cat. No. A300-710A) used for WB at 0.02 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Nucleolin was also immunoprecipitated using rabbit anti-Nucleolin antibodies BL3213 (Cat. No. A300-709A) and BL3215 (Cat. No. A300-711A). Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Detection of human Nucleolin by immunohi

Detection of human Nucleolin by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- Nucleolin (Cat. No. A300-710A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB