Antibody was affinity purified using an epitope specific to p38 MAPK immobilized on solid support. The epitope recognized by A300-708A maps to a region between residue 300 and the C-terminus (residue 360) of human Mitogen Activated Protein Kinase 14 (p38 MAPK) using the numbering given in entry NP_001306.1 (GeneID 1432). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CSAID-binding protein, Csaids binding protein, CSBP, CSBP1, CSBP2, CSPB1, cytokine suppressive anti-inflammatory drug binding protein, Cytokine suppressive anti-inflammatory drug-binding protein, EXIP, MAP kinase 14, MAP kinase Mxi2, MAP kinase p38 alpha,
between 300 and C-term
2 - 8°C
Detection of human and mouse p38 MAPK by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-p38 MAPK antibody BL2226 (Cat. No. A300-708A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). p38 MAPK was also immunoprecipitated using rabbit anti-p38 MAPK antibody BL2225 (Cat. No. A300-707A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).