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Rabbit anti-p38 MAPK Antibody, Affinity Purified

Catalogue number:
A300-707A
Size:
20 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to p38 MAPK immobilized on solid support. The epitope recognized by A300-707A maps to a region between residue 1 and 50 of human Mitogen Activated Protein Kinase 14 (p38 MAPK) using the numbering given in entry NP_001306.1 (GeneID 1432). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

CSAID-binding protein, Csaids binding protein, CSBP, CSBP1, CSBP2, CSPB1, cytokine suppressive anti-inflammatory drug binding protein, Cytokine suppressive anti-inflammatory drug-binding protein, EXIP, MAP kinase 14, MAP kinase Mxi2, MAP kinase p38 alpha,

Species Reactivity:

Human, Mouse

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse p38 MAPK by

Detection of human and mouse p38 MAPK by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-p38 MAPK antibody BL2225 (Cat. No. A300-707A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). p38 MAPK was also immunoprecipitated using rabbit anti-p38 MAPK antibody BL2226 (Cat. No. A300-708A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).