Antibody was affinity purified using an epitope specific to HDAC2 immobilized on solid support. The epitope recognized by A300-705A maps to a region between residue 425 and the C-terminus (residue 488) of human Histone Deacetylase 2 using the numbering given in entry NP_001518.1 (GeneID 3066). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
HD2, histone deacetylase 2, RPD3, transcriptional regulator homolog RPD3, YAF1, YY1-associated factor 1
between 425 and C-term
2 - 8°C
Detection of human and mouse HDAC2 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HDAC2 antibody BL2667 (Cat. No. A300-705A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). HDAC2 was also immunoprecipitated using rabbit anti-HDAC2 antibody BL2666 (Cat. No. A300-704A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Detection of human HDAC2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HDAC2 (Cat. No. A300-705A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB