Antibody was affinity purified using an epitope specific to CDX2 immobilized on solid support. The epitope recognized by A300-691A maps to a region between residue 250 and 300 of human Caudal Type Homeobox Transcription Factor 2 using the numbering given in entry NP_001256.1 (GeneID 1045). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
caudal type homeobox transcription factor 2, caudal-type homeobox protein 2, CDX2/AS, CDX3, CDX-3, homeobox protein CDX-2, homeobox protein miniCDX2
between 250 and 300
2 - 8°C
Detection of human CDX2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CDX2 antibody BL3194 (Cat. No. A300-691A) used for IP at 3 µg/mg lysate. CDX2 was also immunoprecipitated by rabbit anti-CDX2 antibody BL3195 (Cat. No. A300-692A) using 3 µg/mg lysate. For blotting immunoprecipitated CDX2, BL3195 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human CDX2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CDX2 (Cat. No. A300-691A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB