Antibody was affinity purified using an epitope specific to Sirt1 immobilized on solid support. The epitope recognized by A300-687A maps to a region between residue 25 and 75 of human Sirtuin (Silent Mating Type Information Regulation 2) type 1 using the numbering given in entry NP_036370.2 (GeneID 23411). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
hSIR2, hSIRT1, NAD-dependent protein deacetylase sirtuin-1, regulatory protein SIR2 homolog 1, SIR2, SIR2alpha, SIR2L1, SIR2-like protein 1, sirtuin type 1
between 25 and 75
2 - 8°C
Detection of human Sirt1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Sirt1 antibody BL3200 (Cat. No. A300-687A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Sirt1 was also immunoprecipitated using rabbit anti-Sirt1 antibodies BL3201 and BL3203 (Cat. No. A300-688A). Detection: Chemiluminescence with exposure times of 10 seconds (A) and 30 seconds (B).