Antibody was affinity purified using an epitope specific to MAML2 immobilized on solid support. The epitope recognized by A300-682A maps to a region between residue 1100 and the C-terminus (residue 1153) of human Mastermind-Like 2 using the numbering given in entry AAK93831.1 (GeneID 84441). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
MAM2, mam-2, MAM3, MAM-3, mastermind-like 2, mastermind-like protein 2, MLL-MAML2
between 1100 and C-term
2 - 8°C
Detection of human and mouse MAML2 by western blot (h and m) and immunoprecipitation (m). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MAML2 antibody BL1242 (Cat. No. A300-682A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. MAML2 was also immunoprecipitated by rabbit anti-MAML2 antibodies BL1240 and BL1241 (Cat. No. A300-681A). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of human MAML2 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MAML2 (Cat. No. A300-682A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB