Antibody was affinity purified using an epitope specific to GbetaL immobilized on solid support. The epitope recognized by A300-679A maps to a region between residues 275 and the C-terminus (residue 326) of human G protein Beta subunit-like using the numbering given in entry NP_071767.2 (GeneID 64223). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
G protein beta subunit-like, gable, GbetaL, GBL, LST8, mammalian lethal with SEC13 protein 8, mLST8, POP3, protein GbetaL, target of rapamycin complex subunit LST8, TORC subunit LST8, WAT1
between 275 and C-term
2 - 8°C
Detection of human GbetaL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GbetaL antibody A300-679A (lot A300-679A-2) used for IP at 6 µg per reaction. GbetaL was also immunoprecipitated by rabbit anti-GbetaL antibody A300-680A. For blotting immunoprecipitated GbetaL, A300-680A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.