Antibody was affinity purified using an epitope specific to hnRNP-K immobilized on solid support. The epitope recognized by A300-676A maps to a region between residue 300 and 350 of human Heterogeneous Nuclear Ribonucleoprotein K using the numbering given in entries NP_002131.2 for hnRNP-K isoform a and NP_112552.1 for hnRNP-K isoform b (GeneID 3190). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
AUKS, CSBP, dC-stretch binding protein, heterogeneous nuclear ribonucleoprotein K, hnRNP K, HNRPK, transformation upregulated nuclear protein, Transformation up-regulated nuclear protein, TUNP
between 300 and 350
2 - 8°C
Detection of human and mouse hnRNP-K by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-hnRNP-K antibody BL3185 (Cat. No. A300-676A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. immunoprecipitation reactions were also performed using antibodies BL3183 (Cat. No. A300-674A), BL3184 (Cat. No. A300-675A), BL3186 (Cat. No. A300-677A) and BL3187 (Cat. No. A300-678A). Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Detection of human and mouse hnRNP-K by immunohistochemistry. Sample: FFPE section of human colon carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-hnRNP-K (Cat. No. A300-676A Lot1) used at a dilution of 1:200 (1µg/ml) and 1:1,000 (0.2µg/ml). Detection: DAB