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Rabbit anti-REA Antibody, Affinity Purified - A300-657A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-REA Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-657A
Name:
Rabbit anti-REA Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to re immobilized on solid support.The epitope recognized by A300-657A maps to a region between residues 225 and 275 of human Repressor of Estrogen Activity (Prohibitin 2) using the numbering given in entry NP_009204.1 (GeneID 11331).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
BAP, Bap37, BCAP37, B-cell associated protein, B-cell receptor-associated protein BAP37, D-prohibitin, hBAP, p22, PNAS-141, prohibitin-2, REA, repressor of estrogen receptor activity
Species Reactivity:
Human, Mouse
Immunogen:
Between 225 and 275
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse REA by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-REA antibody A300-657A (lot A300-657A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human REA by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-REA antibody A300-657A (lot A300-657A-2) used for IP at 6 µg per reaction. REA was also immunoprecipitated by rabbit anti-REA antibody A300-656A. For blotting immunoprecipitated REA, A300-657A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

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