Antibody was affinity purified using an epitope specific to DDX20 immobilized on solid support. The epitope recognized by A300-651A maps to a region between residues 675 and 725 of human DEAD (Asp-Glu-Ala-Asp) Box 20 using the numbering given in entry NP_009135.3 (GeneID 11218). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
component of gems 3, DEAD (Asp-Glu-Ala-Asp) box polypeptide 20, DEAD box protein 20, DEAD box protein DP 103, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD, DEAD-box protein DP103, DP103, GEMIN3, gemin-3, probable ATP-dependent RNA helicase DDX20
between 675 and 725
2 - 8°C
Detection of human DDX20 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX20 antibody (Cat. No. A300-651A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. DDX20 was also immunoprecipitated using rabbit anti-DDX20 antibodies BL3068, BL3069 (Cat. No. A300-650A) and BL3071, each of which recognizes a different site on DDX20. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).