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Rabbit anti-DEC1 Antibody, Affinity Purified - A300-649A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-DEC1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-649A
Name:
Rabbit anti-DEC1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to DEC1 immobilized on solid support.The epitope recognized by A300-649A maps to a region between residues 350 and the C-terminus (residue 412) of human Differentially Expressed in Chondrocytes (Basic Helix-Look-Helix Domain Containing, Class B, 2) using the numbering given in entry NP_003661.1 (GeneID 8553).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
DEC1, basic helix-loop-helix domain containing, class B, 2, BHLHB2, bHLHe40, class B basic helix-loop-helix protein 2, class E basic helix-loop-helix protein 40, Clast5, differentially expressed in chondrocytes 1, differentially expressed in chondrocytes
Species Reactivity:
Human
Immunogen:
Between 350 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human DEC1 by western blot. Samples: Whole cell lysate (50, 15 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DEC1 antibody A300-649A (lot A300-649A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human DEC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DEC1 antibody A300-649A (lot A300-649A-2) used for IP at 6 µg per reaction. DEC1 was also immunoprecipitated by rabbit anti-DEC1 antibody BL2927. For blotting immunoprecipitated DEC1, A300-649A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human DEC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DEC1 (Cat. No. A300-649A Lot2) used at a dilution of 1:1,000 ( 1µg/ml). Detection: DAB

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