Antibody was affinity purified using an epitope specific to GAPDH immobilized on solid support. The epitope recognized by A300-643A maps to a region between residues 300 and the C-terminus (residue 335) of mouse Glyceraldehyde-3-Phosphate Dehydrogenase using the numbering given in entry NP_032110.1 (GeneID 14433). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 eq5als 1.0 mg of IgG.78
Gapd, GAPDH, glyceraldehyde-3-phosphate dehydrogenase, peptidyl-cysteine S-nitrosylase GAPDH
between 300 and C-term
2 - 8°C
Detection of mouse GAPDH by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody A300-643A (lot A300-643A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (Cat. No. A300-643A Lot 2) used at a dilution of 1:1000 (0.2µg/ml). Detection: DAB