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Rabbit anti-GAPDH Antibody, Affinity Purified

Catalogue number:
A300-643A
Size:
20 µg
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Antibody was affinity purified using an epitope specific to GAPDH immobilized on solid support. The epitope recognized by A300-643A maps to a region between residues 300 and the C-terminus (residue 335) of mouse Glyceraldehyde-3-Phosphate Dehydrogenase using the numbering given in entry NP_032110.1 (GeneID 14433). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 eq5als 1.0 mg of IgG.78

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

Gapd, GAPDH, glyceraldehyde-3-phosphate dehydrogenase, peptidyl-cysteine S-nitrosylase GAPDH

Species Reactivity:

Mouse

Immunogen:

between 300 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of mouse GAPDH by western blot

Detection of mouse GAPDH by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody A300-643A (lot A300-643A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of mouse GAPDH by immunohistoc

Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (Cat. No. A300-643A Lot 2) used at a dilution of 1:1000 (0.2µg/ml). Detection: DAB