Antibody was affinity purified using an epitope specific to GAPDH immobilized on solid support. The epitope recognized by A300-640A maps to a region between residues 200 and 250 of human Glyceraldehyde-3-Phosphate Dehydrogenase using the numbering given in entry NP_002037.2 (GeneID 2597). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, G3PD, GAPD, GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HEL-S-162eP, OCAS, p38 component, Oct1 coactivator in S phase, 38 Kd component, peptidyl-cysteine S-nitrosyl
between 200 and 250
2 - 8°C
Detection of human and mouse GAPDH by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and mouse NIH 3T3 (M; 50 µg). Antibody: Affinity purified rabbit anti-GAPDH antibody (Cat. No. A300-640A) used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.