Antibody was affinity purified using an epitope specific to SKIV2L2 immobilized on solid support. The epitope recognized by A300-614A maps to a region between residues 100 and 150 of human SuperKiller Viralicidic Activity 2-Like 2 using the numbering given in entry NP_056175.2 (GeneID 23517). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent helicase SKIV2L2, ATP-dependent RNA helicase DOB1, ATP-dependent RNA helicase SKIV2L2, Dob1, exosome RNA helicase MTR4, fSAP118, functional spliceosome-associated protein 118, KIAA0052, Mtr4, Ski2 like RNA helicase 2, SKIV2L2, superkiller vi
between 100 and 150
2 - 8°C
Detection of human and mouse SKIV2L2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SKIV2L2 antibody A300-614A (lot A300-614A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human SKIV2L2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SKIV2L2 antibody A300-614A (lot A300-614A-3) used for IP at 3 µg per reaction. SKIV2L2 was also immunoprecipitated by rabbit anti-SKIV2L2 antibody A300-615A. For blotting immunoprecipitated SKIV2L2, A300-614A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human SKIV2L2 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-SKIV2L2 (Cat. No. A300-614A Lot3) used at a dilution of 1:200 (1µg/ml). Detection: DAB