Rabbit anti-MafA Antibody, Affinity Purified - A300-611A - 100 µg - Bethyl Laboratories, Inc. - antibodies
The epitope recognized by A300-611A maps to a region between residue 300 and the C-terminus (residue 359) of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog A using the numbering given in entry NP_919331.1 (GeneID 378435). Antibody was affinity purified using the peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB
Detection of MafA, MafB and cMaf by western blot. Samples: Nuclear extract (6 µg) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069 (Cat. No. A300-611A), anti-MafB Antibody (Cat. No. A300-612A) or anti-c-Maf antibody BL662 (Cat. No. A300-613A). Each antibody was used at 0.5 µg/ml. Detection: Chemiluminescence with a 5 second exposure.
Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 µg) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069 (Cat. No. A300-611A), anti-MafB Antibody BL658 (Cat. No. A300-612A) or anti-c-Maf antibody BL662 (Cat. No. A300-613A).
Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: immunoprecipitatesd cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, reactions were run with no DNA, with input chromatin, with DNA obtained after precipitation with rabbit IgG or without antibody (lanes 1 through 5, respectively). Antibody: Affinity purified rabbit anti-MafA antibody BL1069 (Cat. No. A300-611A).
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