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Rabbit anti-MafA Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£445.00 Estimated delivery Fri 3 May
Shipping is calculated in checkout
Applications:

WB

Antibody Host:

Rabbit

Target:

MafA

Species Reactivity:

Mouse

Antibody Type:

Polyclonal

Immunogen:

between 300 and C-term

Alternative Names:

Mafa homolog; pancreatic beta-cell-specific transcriptional activator; RIPE3b1; transcription factor MafA; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A

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Product Description:

Rabbit anti-MafA Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of MafA, MafB and cMaf by west
Detection of MafA, MafB and cMaf by western blot. Samples: Nuclear extract (6 µg) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069 (Cat. No. A300-611A), anti-MafB Antibody (Cat. No. A300-612A) or anti-c-Maf antibody BL662 (Cat. No. A300-613A). Each antibody was used at 0.5 µg/ml. Detection: Chemiluminescence with a 5 second exposure.
Electrophoretic Mobility Shift of MafA,
Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 µg) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069 (Cat. No. A300-611A), anti-MafB Antibody BL658 (Cat. No. A300-612A) or anti-c-Maf antibody BL662 (Cat. No. A300-613A).
Binding of MafA to the enhancer region o
Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: immunoprecipitatesd cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, reactions were run with no DNA, with input chromatin, with DNA obtained after precipitation with rabbit IgG or without antibody (lanes 1 through 5, respectively). Antibody: Affinity purified rabbit anti-MafA antibody BL1069 (Cat. No. A300-611A).
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