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Rabbit anti-VCP Antibody, Affinity Purified

Catalogue number:
A300-588A
Size:
20 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to VCP immobilized on solid support. The epitope recognized by A300-588A maps to a region between residues 1 and 50 of human Valosin-Containing Protein using the numbering given in entry NP_009057.1 (GeneID 7415). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

15S Mg(2+)-ATPase p97 subunit, CDC48, p97, TER ATPase, TERA, transitional endoplasmic reticulum ATPase, Valosin-containing protein, VCP

Species Reactivity:

Human, Mouse

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse VCP by west

Detection of human and mouse VCP by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-VCP antibody A300-588A (lot A300-588A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human VCP by western blot o

Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody A300-588A (lot A300-588A-2) used for IP at 3 µg per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody A300-589A. For blotting immunoprecipitated VCP, A300-588A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.