Antibody was affinity purified using an epitope specific to NONO immobilized on solid support. The epitope recognized by A300-582A maps to a region between residues 1 and 50 of human Non-POU Domain Containing, Octamer-binding using the numbering given in entry NP_031389.3 (GeneID 4841). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, DNA-binding p52/p100 complex, 52 kDa subunit, MRXS34, NMT55, NonO protein, non-POU domain-containing octamer (ATGCAAAT) binding protein, non-POU domain-containing octamer-binding protein
between 1 and 50
2 - 8°C
Detection of human NONO by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 1/4 of IP reaction loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NONO antibody BL2552 (Cat. No. A300-582A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NONO was also immunoprecipitated using anti-NONO antibody BL2253 (Cat. No. A300-587A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).