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Rabbit anti-S6K2 Antibody, Affinity Purified - A300-576A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-S6K2 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-576A
Name:
Rabbit anti-S6K2 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to S6K2 immobilized on solid support. The epitope recognized by A300-576A maps to a region between residue 430 and the C-terminus (residue 482) of human Ribosomal Protein S6 Kinase, 70kda, polypeptide 2 using the numbering given in entry NP_003943.2 (GeneID 6199).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
70 kDa ribosomal protein S6 kinase 2, KLS, p70 ribosomal S6 kinase beta, p70 S6 kinase beta, p70 S6KB, p70 S6K-beta, p70(S6K)-beta, P70-beta, P70-beta-1, P70-beta-2, p70-S6K 2, P70S6K2, p70S6Kb, ribosomal protein S6 kinase beta-2, ribosomal protein S6 kin
Species Reactivity:
Human, Mouse
Immunogen:
Between 430 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse S6K2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-S6K2 antibody A300-576A (lot A300-576A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human S6K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-S6K2 antibody A300-576A (lot A300-576A-2) used for IP at 3 µg per reaction. S6K2 was also immunoprecipitated by rabbit anti-S6K2 antibody A300-575A. For blotting immunoprecipitated S6K2, A300-576A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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