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Rabbit anti-S6K2 Antibody, Affinity Purified

Catalogue number:
A300-576A
Size:
20 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to S6K2 immobilized on solid support. The epitope recognized by A300-576A maps to a region between residue 430 and the C-terminus (residue 482) of human Ribosomal Protein S6 Kinase, 70kda, polypeptide 2 using the numbering given in entry NP_003943.2 (GeneID 6199). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

70 kDa ribosomal protein S6 kinase 2, KLS, p70 ribosomal S6 kinase beta, p70 S6 kinase beta, p70 S6KB, p70 S6K-beta, p70(S6K)-beta, P70-beta, P70-beta-1, P70-beta-2, p70-S6K 2, P70S6K2, p70S6Kb, ribosomal protein S6 kinase beta-2, ribosomal protein S6 kin

Species Reactivity:

Human, Mouse

Immunogen:

between 430 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse S6K2 by wes

Detection of human and mouse S6K2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-S6K2 antibody A300-576A (lot A300-576A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human S6K2 by western blot

Detection of human S6K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-S6K2 antibody A300-576A (lot A300-576A-2) used for IP at 3 µg per reaction. S6K2 was also immunoprecipitated by rabbit anti-S6K2 antibody A300-575A. For blotting immunoprecipitated S6K2, A300-576A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.