Antibody was affinity purified using an epitope specific to S6K2 immobilized on solid support. The epitope recognized by A300-576A maps to a region between residue 430 and the C-terminus (residue 482) of human Ribosomal Protein S6 Kinase, 70kda, polypeptide 2 using the numbering given in entry NP_003943.2 (GeneID 6199). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
70 kDa ribosomal protein S6 kinase 2, KLS, p70 ribosomal S6 kinase beta, p70 S6 kinase beta, p70 S6KB, p70 S6K-beta, p70(S6K)-beta, P70-beta, P70-beta-1, P70-beta-2, p70-S6K 2, P70S6K2, p70S6Kb, ribosomal protein S6 kinase beta-2, ribosomal protein S6 kin
between 430 and C-term
2 - 8°C
Detection of human and mouse S6K2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-S6K2 antibody A300-576A (lot A300-576A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human S6K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-S6K2 antibody A300-576A (lot A300-576A-2) used for IP at 3 µg per reaction. S6K2 was also immunoprecipitated by rabbit anti-S6K2 antibody A300-575A. For blotting immunoprecipitated S6K2, A300-576A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.