Antibody was affinity purified using an epitope specific to BRIP1/BACH1 immobilized on solid support. The epitope recognized by A300-560A maps to a region between residues 1100 and 1150 of human Brca1 Interacting Protein C-terminal Helicase 1 using the numbering given in entry NP_114432.1 (GeneID 83990). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase BRIP1, BACH1, BRCA1/BRCA2-associated helicase 1, BRCA1-associated C-terminal helicase 1, BRCA1-binding helicase-like protein BACH1, BRCA1-interacting protein 1, BRCA1-interacting protein C-terminal helicase 1, FANCJ, Fanconi ane
between 1100 and 1150
2 - 8°C
Detection of human BRIP1(BACH1) by immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 1/4 of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-BRIP1(BACH1) antibody BL2599 (Cat. No. A300-560A) used for IP at 3 µg/mg lysate. BRIP1(BACH1) was also immunoprecipitated using rabbit anti-BRIP1(BACH1) antibody BL2600 (Cat. No. A300-561A), which is to a downstream epitope, at 3 µg/mg lysate. BRIP1(BACH1) was blotted using BL2600 at 1 µg/ml. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).