Antibody was affinity purified using an epitope specific to ZBTB7/FBI-1 immobilized on solid support. The epitope recognized by A300-549A maps to a region between residue 525 and the C-terminus (residue 584) of human ZBTB7 (Factor Binding IST protein 1) using the numbering given in entry NP_056982.1 (GeneID 51341). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
factor binding IST protein 1, factor that binds to inducer of short transcripts protein 1, FBI1, FBI-1, HIV-1 1st-binding protein 1, HIV-1 inducer of short transcripts binding protein, leukemia/lymphoma-related factor, LRF, lymphoma related factor, POK er
between 525 and C-term
2 - 8°C
Detection of human ZBTB7/FBI-1 by western blot and immunoprecipitation. Samples: A) Whole cell lysate from HeLa (5, 15 and 50 µg), HEK293T (T; 50 µg) and NIH 3T3 (M; 50 µg) cells. B) Whole cell lysate (1 mg for IP; 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody A300-549A used at 0.04 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP (B). ZBTB7/FBI-1 was also immunoprecipitated using rabbit antibodies A300-548A, and BL2686, which are to downstream epitopes on ZBTB7/FBI-1. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Detection of human ZBTB7/FBI-1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- ZBTB7/FBI-1(Cat. No. A300-549A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB