Antibody was affinity purified using an epitope specific to CTCF immobilized on solid support. The epitope recognized by A300-544A maps to a region between residue 700 and the C-terminus (residue 727) of human CCCTC-binding factor using the numbering given in entry NP_006556.1 (GeneID 10664) .Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein), CTCFL paralog, MRD21, transcriptional repressor CTCF
between 700 and C-term
2 - 8°C
immunoprecipitation of human CTCF. Samples: Whole cell lysate (1 mg for IP; 1/4 of IP loaded/lane) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-CTCF antibody BL2547 (Cat. No. A300-544A) used at 3 µg/mg lysate for IP. Human CTCF was also immunoprecipitated using affinity purified rabbit anti-CTCF antibodies BL2545 (Cat. No. A300-542A) and BL2546 (Cat. No. A300-543A). CTCF was blotted using BL2546 at 0.03 µg/ml. Detection: Chemiluminescence with exposure times of 10 seconds.