Antibody was affinity purified using an epitope specific to Pds5B immobilized on solid support. The epitope recognized by A300-537A maps to a region between residues 1200 and 1250 of human Precocious Dissociation of Sisters-5B using the numbering given in entry CAB69911.1 (GeneID 23047). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
androgen induced inhibitor of proliferation, androgen-induced proliferation inhibitor, androgen-induced prostate proliferative shutoff-associated protein AS3, androgen-induced shutoff 3, APRIN, AS3, CG008, sister chromatid cohesion protein PDS5 homolog B
between 1200 and 1250
2 - 8°C
Detection of human and mouse Pds5B by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB in A; 1 mg for IP in B, 1/4 of IP reaction loaded), HEK293T (T, 50 µg for WB) and NIH 3T3 (M, 50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-Pds5B antibody BL2352 (Cat. No. A300-537A) used in WB at 0.04 µg/ml (A) or 0.1 µg/ml (B) and for IP at 3 µg/mg lysate (B). Pds5B was also immunoprecipitated using rabbit anti-Pds5B antibody BL2353 (Cat. No. A300-538A) at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Detection of human Pds5B by immunohistochemistry. Sample: FFPE sections of human skin carcinoma (left) and prostate carcinoma (right). Antibody: Affinity purified rabbit anti- Pds5B (Cat. No. A300-537A Lot1) used at a dilution of 1:200 (1µg/ml) and 1:80 (2.5 µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100