Antibody was affinity purified using an epitope specific to DOCK9 immobilized on solid support. The epitope recognized by A300-532A maps to a region between residues 1850 and 1900 of human Dedicator of Cytokinesis 9 using the numbering given in entry NP_056111.1 (GeneID 23348). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
cdc42 guanine nucleotide exchange factor zizimin-1, dedicator of cytokinesis protein 9, ZIZ1, ZIZIMIN1, zizimin-1
between 1850 and 1900
2 - 8°C
Detection of human and mouse DOCK9 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DOCK9 antibody A300-532A (lot A300-532A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human DOCK9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DOCK9 antibody A300-532A (lot A300-532A-2) used for IP at 6 µg per reaction. DOCK9 was also immunoprecipitated by a previous lot of this antibody (lot A300-532A-1) and rabbit anti-DOCK9 antibodies A300-530A and A300-531A. Detection: Chemiluminescence with an exposure time of 3 minutes.