Antibody was affinity purified using an epitope specific to TSC2 immobilized on solid support. The epitope recognized by A300-526A maps to a region between residues 1 and 50 of human Tuberous Sclerosis 2 using the numbering given in Swiss-Prot entry P49815 (GeneID 7249). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: IP
LAM, PPP1R160, protein phosphatase 1, regulatory subunit 160, TSC4, tuberin, tuberous sclerosis 2 protein
between 1 and 50
2 - 8°C
Detection of human TSC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TSC2 antibody A300-526A (lot A300-526A-2) used for IP at 3 µg per reaction. TSC2 was also immunoprecipitated by rabbit anti-TSC2 antibody A300-463A. For blotting immunoprecipitated TSC2, A300-463A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.