Antibody was affinity purified using an epitope specific to DDX5 immobilized on solid support. The epitope recognized by A300-523A maps to a region between residue 575 and the C-terminus (residue 614) of human DEAD (Asp-Glu-Ala-Asp) Polypeptide 5 using the numbering given in entry NP_004387.1 (GeneID 1655). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase DDX5, DEAD (Asp-Glu-Ala-Asp) box helicase 5, DEAD (Asp-Glu-Ala-Asp) box polypeptide 5, DEAD box protein 5, DEAD box-5, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD), G17P1, HLR1, HUMP68, p68, probable ATP-d
between 575 and C-term
2 - 8°C
Detection of human and mouse DDX5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX5 antibody A300-523A (lot A300-523A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Detection of human DDX5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX5 antibody A300-523A (lot A300-523A-2) used for IP at 6 µg per reaction. DDX5 was also immunoprecipitated by a previous lot of this antibody (lot A300-523A-1) and rabbit anti-DDX5 antibody A300-522A For blotting immunoprecipitated DDX5, A300-523A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.