Antibody was affinity purified using an epitope specific to 4EBP1 immobilized on solid support. The epitope recognized by A300-501A maps to a region between residue 75 and the C-terminus (residue 118) of human eukaryotic translation initiation factor 4E binding protein 1 using the numbering given in entry NP_004086.1 (GeneID 1978). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
4EBP1, 4E-BP1, BP-1, eIF4E-binding protein 1, eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, phosphorylated heat- and acid-stable protein regulated by insulin 1
between 75 and C-term
2 - 8°C