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Rabbit anti-4EBP1 Antibody, Affinity Purified

Catalogue number:
A300-501A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to 4EBP1 immobilized on solid support. The epitope recognized by A300-501A maps to a region between residue 75 and the C-terminus (residue 118) of human eukaryotic translation initiation factor 4E binding protein 1 using the numbering given in entry NP_004086.1 (GeneID 1978). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IHC
Product Type:

Antibodies

Alternative Names:

4EBP1, 4E-BP1, BP-1, eIF4E-binding protein 1, eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, phosphorylated heat- and acid-stable protein regulated by insulin 1

Species Reactivity:

Human

Immunogen:

between 75 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of 4EBP1 by western blot. Samp

Detection of 4EBP1 by western blot. Samples: Whole cell lysate (100 µg) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody: Affinity purified rabbit anti-4EBP1 (Cat. No. A300-501A) used at 0.25 µg/ml. Detection: Chemiluminescence.

Detection of human 4EBP1 by immunohistoc

Detection of human 4EBP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 (Cat. No. A300-501A) used at a dilution of 1:20,000 ( 0.05µg/ml). Detection: DAB