Antibody was affinity purified using an epitope specific to SLK immobilized on solid support. The epitope recognized by A300-499A maps to a region between residues 1 and 50 of human Ste20-like Kinase using the numbering given in entry NP_055535.2 (GeneID 9748). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
bA16H23.1, CTCL tumor antigen se20-9, hSLK, Long Ste20-like Kinase, LOSK, se20-9, serine/threonine-protein kinase 2, SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase, SNF1 sucrose nonfermenting like kinase, SNF1 sucrose nonfermenting like kinase,
between 1 and 50
2 - 8°C
Detection of human SLK by western blot and immunoprecipitation. Samples: A) Whole cell lysate from MCF-7 (5, 15 and 50 µg), HeLa (H; 15 µg) and HEK293T (T; 15 µg). B) Whole cell lysate (1 mg/IP; 1/4 of IP loaded) from MCF7 cells. Antibodies: Affinity purified rabbit anti-SLK antibody BL1915 (Cat. No. A300-499A) used at 1 µg/ml for WB (A and B) and at 3 µg/mg lysate for IP (B). SLK was also efficiently immunoprecipitated by BL1917 (Cat. No. A300-500A) but not by BL1916. Detection: Chemiluminescence with an exposure time of 3 minutes (A and B).