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Rabbit anti-Lasu1/Ureb1 Antibody, Affinity Purified - A300-486A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Lasu1/Ureb1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-486A
Name:
Rabbit anti-Lasu1/Ureb1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Ureb1/Lasu1 immobilized on solid support. The epitope recognized by A300-486A maps to a region between residues 2250 and 2300 of human Upstream Regulatory Element Binding Protein 1 using the numbering given in entry NP_113584.3 (GeneID 10075). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
ARF-binding protein 1, ARF-BP1, BJ-HCC-24 tumor antigen, E3 ubiquitin-protein ligase HUWE1, HECT domain protein LASU1, HECT, UBA and WWE domain-containing protein 1, HECTH9, HECT-type E3 ubiquitin transferase HUWE1, homologous to E6AP carboxyl terminus ho
Species Reactivity:
Human
Immunogen:
Between 2250 and 2300
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Lasu1/Ureb1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 antibody A300-486A (lot A300-486A-9) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

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Detection of human Lasu1/Ureb1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Lasu1/Ureb1 antibody A300-486A (lot A300-486A-9) used for IP at 6 µg per reaction. Lasu1/Ureb1 was also immunoprecipitated by a previous lot of this antibody (lot A300-486A-8). For blotting immunoprecipitated Lasu1/Ureb1, A300-486A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

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Detection of human Lasu1/Ureb1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 (Cat. No. A300-486A Lot 9) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.

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