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Rabbit anti-Lasu1/Ureb1 Antibody, Affinity Purified

Catalogue number:
A300-486A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to Ureb1/Lasu1 immobilized on solid support. The epitope recognized by A300-486A maps to a region between residues 2250 and 2300 of human Upstream Regulatory Element Binding Protein 1 using the numbering given in entry NP_113584.3 (GeneID 10075). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

ARF-binding protein 1, ARF-BP1, BJ-HCC-24 tumor antigen, E3 ubiquitin-protein ligase HUWE1, HECT domain protein LASU1, HECT, UBA and WWE domain-containing protein 1, HECTH9, HECT-type E3 ubiquitin transferase HUWE1, homologous to E6AP carboxyl terminus ho

Species Reactivity:

Human

Immunogen:

between 2250 and 2300

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Lasu1/Ureb1 by wester

Detection of human Lasu1/Ureb1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 antibody A300-486A (lot A300-486A-9) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human Lasu1/Ureb1 by wester

Detection of human Lasu1/Ureb1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Lasu1/Ureb1 antibody A300-486A (lot A300-486A-9) used for IP at 6 µg per reaction. Lasu1/Ureb1 was also immunoprecipitated by a previous lot of this antibody (lot A300-486A-8). For blotting immunoprecipitated Lasu1/Ureb1, A300-486A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human Lasu1/Ureb1 by immuno

Detection of human Lasu1/Ureb1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 (Cat. No. A300-486A Lot 9) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.