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Rabbit anti-Rictor Antibody, Affinity Purified - A300-459A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Rictor Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-459A
Name:
Rabbit anti-Rictor Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Rictor immobilized on solid support. The epitope recognized by A300-459A maps to a region between residue 1650 and the C-terminus (residue 1708) of human Rapamycin-Insensitive Companion of mTOR using the numbering given in TrEMBL entry Q6R327 (GeneID 253260).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
AVO3, AVO3 homolog, hAVO3, PIA, pianissimo, rapamycin-insensitive companion of mTOR, TORC2-specific protein AVO3
Species Reactivity:
Human, Mouse
Immunogen:
Between 1650 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Rictor by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody A300-459A (lot A300-459A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of mouse Rictor by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody A300-459A (lot A300-459A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rictor antibody A300-459A (lot A300-459A-4) used for IP at 3 µg per reaction. Rictor was also immunoprecipitated by a previous lot of this antibody (lot A300-459A-3) and rabbit anti-Rictor antibody A300-458A. For blotting immunoprecipitated Rictor, A300-459A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human Rictor by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Rictor (Cat. No. A300-459A Lot 4) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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