Antibody was affinity purified using an epitope specific to EWS immobilized on solid support. The epitope recognized by A300-416A maps to a region between residues 1 and 50 of human Ewing sarcoma breakpoint region 1 using the numbering given in SwissProt entry Q01844 (GeneID 2130). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
bK984G1.4, Ewing sarcoma breakpoint region 1, Ewing sarcoma breakpoint region 1 protein, Ewings sarcoma EWS-Fli1 (type 1) oncogene, EWS, EWS oncogene, EWS RNA-binding protein variant 6, EWS-FLI1, RNA-binding protein EWS
between 1 and 50
2 - 8°C
Detection of human EWS by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody A300-416A (lot A300-416A-1) used for WB at 0.066 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.