Antibody was affinity purified using an epitope specific to HdmX/MDM4 phosphorylated at Ser 403 immobilized on solid support. The epitope recognized by A300-392A maps to a region surrounding phosphorylated Ser 403 of human ortholog of mouse double minute 4 using the numbering given in entry NP_002384.2 (GeneID 4194).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Double minute 4 protein, double minute 4, human homolog of, HDMX, mdm2-like p53-binding protein, Mdm4 p53 binding protein homolog, MDM4 protein variant G, MDM4 protein variant Y, MDM4-related protein 1, MDMX, MRP1, p53-binding protein, p53-binding protein
Surrounding serine 403
2 - 8°C
Detection of phospho HdmX/MDM4 (Ser 403) by western blot. Samples: Whole cell lysate (30 µg/lane) from HEK293T cells that were mock transfected, or transfected with expression constructs for HA tagged wild-type HdmX/MDM4 or HA tagged HdmX/MDM4 (S403A) mutant. Antibody: Affinity purified rabbit anti-phospho HdmX/MDM4 antibody (Cat. No. A300-392A) used at 0.13 µg/ml for WB. Detection: Chemiluminescence with an exposure time of 30 seconds.