Antibody was affinity purified using an epitope specific to BNF1 immobilized on solid support. The epitope recognized by A300-378A maps to a region between residues 200 and 250 of human breast tumor novel factor 1 using the numbering given in TrEMBL entry Q7Z5J3 (GeneID 25884). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB
BNF1, BNF-1, breast tumor novel factor 1, CHL2, chordin-like protein 2, chordin-related protein 2
between 200 and 250
2 - 8°C
Detection of human BNF1 by western blot. Sample: Whole cell lysate (50 µg) from DU145 cells. Antibody: Affinity purified rabbit anti-BNF1 antibody (Cat. No. A300-378A) used at 3 µg/ml. Detection: Chemiluminescence with an exposure time of 1 minute.