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Rabbit anti-eIF3C/eIF3S8 Antibody, Affinity Purified

Catalogue number:
A300-377A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to eIF3C/eIF3S8 immobilized on solid support. The epitope recognized by A300-377A maps to a region between residues 863 and 913 of human eukaryotic translation initiation factor 3, subunit C (eukaryotic translation initiation factor 3, subunit 8, 110kDa) using the numbering given in Swiss-Prot entry Q99613 (GeneID 8663). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

cell migration-inducing protein 17, eIF3 p110, eIF3c, EIF3CL, eIF3-p110, EIF3S8, eukaryotic translation initiation factor 3 subunit 8, eukaryotic translation initiation factor 3 subunit C, eukaryotic translation initiation factor 3, subunit 8 (110kD), euk

Species Reactivity:

Human

Immunogen:

between 850 and 918

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human eIF3C/eIF3S8 by weste

Detection of human eIF3C/eIF3S8 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3C/eIF3S8 antibody A300-377A (lot A300-377A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human eIF3C/eIF3S8 by weste

Detection of human eIF3C/eIF3S8 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF3C/eIF3S8 antibody A300-377A (lot A300-377A-2) used for IP at 6 µg per reaction. eIF3C/eIF3S8 was inefficiently immunoprecipitated by rabbit anti-eIF3C/eIF3S8 antibody BL1724. For blotting immunoprecipitated eIF3C/eIF3S8, A300-377A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.