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Rabbit anti-Bub1 Antibody, Affinity Purified - A300-373A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Bub1 Antibody, Affinity Purified
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Size: 100 µg
£309.00
GBP | Euro | USD
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Catalogue number:
A300-373A
Name:
Rabbit anti-Bub1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Bub1 immobilized on solid support. The epitope recognized by A300-373A maps to a region between residues 1 and 50 human budding uninhibited by benzimidazoles 1 using the numbering given in Swiss-Prot entry O43683 (GeneID 699).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
BUB1 budding uninhibited by benzimidazoles 1 homolog, BUB1A, BUB1L, budding uninhibited by benzimidazoles 1 homolog, hBUB1, mitotic checkpoint serine/threonine-protein kinase BUB1, mitotic spindle checkpoint kinase, putative serine/threonine-protein kinas
Species Reactivity:
Human
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Bub1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa treated with nacodazole (+) or mock treated (-), HEK293T. and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Bub1 antibody A300-373A (lot A300-373A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds

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Detection of human Bub1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with nocodazole. Antibodies: Affinity purified rabbit anti-Bub1 antibody A300-373A (lot A300-373A-2) used for IP at 6 µg per reaction. Bub1 was also immunoprecipitated by rabbit anti-Bub1 antibody BL1683. For blotting immunoprecipitated Bub1, A300-373A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Bub1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Bub1 (Cat. No. A300-373A) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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