Antibody was affinity purified using an epitope specific to BIRC6/Apollon immobilized on solid support. The epitope recognized by A300-367A maps to a region between residue 4775 and the C-terminus (residue 4829) of human Baculoviral IAP repeat-containing protein 6 using the numbering given in Swiss-Prot entry Q9NR09 (GeneID 57448). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
APOLLON, baculoviral IAP repeat-containing protein 6, BIR repeat-containing ubiquitin-conjugating enzyme, BRUCE, RING-type E3 ubiquitin transferase BIRC6, Ubiquitin-conjugating BIR domain enzyme apollon, ubiquitin-conjugating BIR-domain enzyme apollon
between 4775 and C-term
2 - 8°C
Detection of human and mouse BIRC6 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIRC6 antibody A300-367A (lot A300-367A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human BIRC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BIRC6 antibody A300-367A (lot A300-367A-2) used for IP at 6 µg per reaction. BIRC6 was also immunoprecipitated by a previous lot of this antibody (lot A300-367A-1). For blotting immunoprecipitated BIRC6, A300-367A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.