Antibody was affinity purified using an epitope specific to Bub3 immobilized on solid support. The epitope recognized by A300-365A maps to a region between residues 275 and 325 of human Bub3 budding uninhibited by benzimidazoles 3 using the numbering given in Swiss-Prot entry O43684 (GeneID 9184). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BUB3 budding uninhibited by benzimidazoles 3 homolog, BUB3L, budding uninhibited by benomyl, budding uninhibited by benzimidazoles 3 homolog, hBUB3, mitotic checkpoint component, mitotic checkpoint protein BUB3, testicular tissue protein Li 27
between 275 and 325
2 - 8°C
Detection of human Bub3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Bub3 antibody A300-365A (lot A300-365A-1) used for WB at 0.066 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.