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Rabbit anti-Bub3 Antibody, Affinity Purified

Catalogue number:
A300-364A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to Bub3 immobilized on solid support. The epitope recognized by A300-364A maps to a region between residues 1 and 50 of human Bub3 budding uninhibited by benzimidazoles 3 using the numbering given in Swiss-Prot entry O43684 (GeneID 9184). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IHC
Product Type:

Antibodies

Alternative Names:

BUB3 budding uninhibited by benzimidazoles 3 homolog, BUB3L, budding uninhibited by benomyl, budding uninhibited by benzimidazoles 3 homolog, hBUB3, mitotic checkpoint component, mitotic checkpoint protein BUB3, testicular tissue protein Li 27

Species Reactivity:

Human

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Bub3 by western blot.

Detection of human Bub3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Bub3 antibody A300-364A (lot A300-364A-1) used for WB at 0.066 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Bub3 by immunohistoch

Detection of human Bub3 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Bub3 (Cat. No. A300-364A) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB