Antibody was affinity purified using an epitope specific to CBP immobilized on solid support. The epitope recognized by A300-363A maps to a region between residues 2375 and 2425 of human CREB Binding Protein using the numbering given in entry NP_004371.1 (GeneID 1387). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CBP, CREB-binding protein, KAT3A, RSTS, RSTS1
between 2375 and 2425
2 - 8°C
Detection of human CBP by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody A300-363A (lot A300-363A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human CBP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody A300-363A (lot A300-363A-1) used for IP at 6 µg/mg lysate. CBP was also immunoprecipitated by rabbit anti-CBP antibody A300-362A. For blotting immunoprecipitated CBP, A300-363A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human CBP by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBP (Cat. No. A300-363A) used at a dilution of 1:500 (2µg/ml). Detection: DAB